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Shpacovitch/etal/2015a: Application of Surface Plasmon Resonance Imaging Technique for the Detection of Single Spherical Biological Submicron-particles

Bibtype Article
Bibkey Shpacovitch/etal/2015a
Author Shpacovitch, Victoria and Temchura, Vladimir and Matrosovich, Mikhail and Hamacher, Joachim and Skolnik, Julia and Libuschewski, Pascal and Siedhoff, Dominic and Weichert, Frank and Marwedel, Peter and Müller, Heinrich and Überla, Klaus and Hergenröder, Roland and Zybin, Alexander
Title Application of Surface Plasmon Resonance Imaging Technique for the Detection of Single Spherical Biological Submicron-particles
Journal Analytical Biochemistry: Methods in the Biological Sciences
Abstract Recent proof-of-principle studies demonstrated the suitability of surface plasmon resonance imaging (SPRi) technique for the detection of individual submicron- and nano-particles in solutions. In the current study, we used the SPRi technique for visualization the binding of round-shaped viruses (inactivated influenza A virus) and virus-like particles (human immunodeficiency virus (HIV)-based virus-like particles) to the functionalized sensor surface. We show the applicability of the SPRi technique for the detection of individual virus-like particles in buffers without serum as well as in buffers containing different concentrations of serum. Furthermore, we prove the specificity of visualized binding events using two different pseudotypes of HIV virus-like particles. We also demonstrate the applicability of the SPRi technique for the determination of relative particle concentrations in solutions. Moreover, we suggest a technical approach, which allows enhancing the magnitude of binding signals. Our studies indicate that the SPRi technique represents an efficient research tool for quantification and characterization of biological submicron-objects such as viruses or virus-like particles, for example.
Year 2015
Projekt SFB876-B2
 


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